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刘文俊

添加时间:2011年11月12日 17:24 来源: 作者: 浏览次数:

刘文俊

博士,副研究员

通讯地址

“乳品生物技术与工程”教育部重点实验室,内蒙古农业大学   

赛罕区昭乌达路306号   

中国内蒙古自治区呼和浩特市,010018   

Tel: 86-471-4308703;Fax: 86-471-4305357   

Email:wjliu168@163.com

Web site:www.dairy-biotics.cn

 

 

 

 

 

 

教育背景

1999-2003年,内蒙古民族大学,农学院,获农学专业学士学位.本科论文:低磷胁迫对大豆生理及形态指标的影响.   

2003-2007年,华中农业大学,生命科学技术学院,获生化与分子生物学专业理学硕士学位.硕士论文:稻米粗脂肪含量QTL定位及其遗传基础研究.   

2010-2014年,内蒙古农业大学,食品科学与工程学院,获农产品贮藏与加工工程专业博士学位。博士论文题目:嗜热链球菌和保加利亚乳杆菌产酸、风味特性及其功能基因分型和表达研究   

工作经历

2007年7月-2009年10月,内蒙古农业大学食品科学与工程学院,实习研究员   

2009年10月-2016年7月,内蒙古农业大学食品科学与工程学院,助理研究员   

2016年7月-至今,内蒙古农业大学食品科学与工程学院,副研究员   

自入职以来,一直在内蒙古农业大学乳品生物技术与工程教育部重点实验室工作,主要从事乳酸菌生物技术与分子生物学方面的研究,围绕传统发酵乳制品和乳酸菌的分离鉴定取得如下研究成果:   

主持的科研项目

1.基于基因组尺度代谢网络重构对乳杆菌高密度发酵分子机制的研究,国家自然科学基金(面上)项目(2016年1月-2020年12月),62.0万元,(项目号:31671871)。主持人

2.自然发酵乳制品制作过程中微生物群落和功能多样性动态研究,国家自然科学基金(青年)项目(2014年1月-2016年12月),24万元,(项目号:31301518)。   

3.内蒙古传统酸马奶发酵过程中细菌群落结构动态变化及共研究,内蒙古自然科学基金面上项目(2016年1月-2019年12月),8.0万元,(项目编号:2016MS0316)。主持人

4.德氏乳杆菌保加利亚亚种后酸化功能基因定位及其表达调控研究,内蒙自然科学基金面上项目(2013年1月-2015年12月),5万元,(项目号:2013MS1206)。   

5.“应用宏基因组学方法研究内蒙古地区传统发酵乳制品中乳酸菌生物多样性”,内蒙古自治区自然科学基金项目(2009-2011),主持人,3.0万元。(2008MS0409)   

发表文章

在国内外学术期刊发表研究论文50余篇,其中发表第一作者或者通讯作者SCI收录论文16篇.

1.Sun,Z, H M Harris, A McCann, C Guo, S Argimon, W Zhang,W Liu. Expanding the biotechnology potential of lactobacillithrough comparative genomics of 213 strains and associated genera. Nat Commun.2015, 6: 8322. (SCI收录,IF=11.470)

2.Wenjun Liu, Jiachao Zhang, Chunyan Wu, Shunfeng Cai, WeiqiangHuang, Jing Chen, Xiaoxia XI, Zebin Liang, Qiangchuan Hou, Bing Zhou, Nan Qin& Heping Zhang. Unique Features of Ethnic Mongolian Gut Microbiome revealedby metagenomic analysis Scientific Reports, 2016, 6:34826 DOI: 10.1038/srep34826(第一作者,SCI收录,IF=5.228)

3.Wenjun Liu, Jie Yu,Zhihong Sun, Yuqin Song, Xueni Wang, Hongmei Wang, Tuoya Wuren, Musu Zha,Bilige Menghe, and Zhang Heping. Relationships between functional genes inLactobacillus delbrueckii ssp. bulgaricus isolates and phenotypiccharacteristics associated with fermentation time and flavor production inyogurt elucidated using multilocus sequence typing. J. Dairy Sci. 2016, 99(1): 1–15.(第一作者SCI收录,IF=2.573

4.QimuGesudu, Yi Zheng, Xiaoxia Xi, Qiang Chuan Hou, Haiyan Xu, Weiqiang Huang,Wenjun Liu* Investigating bacterial population structure and dynamics intraditional koumiss from Inner Mongolia using single molecule real-timesequencing. J. Dairy Sci. 2016, 99:7852–7863(通讯作者,SCI收录,IF=2.573)

5.Wenjun Liu, Yi Zheng, Lai-Yu Kwok,Zhihong Sun, Jiachao Zhang, Zhuang Guo, Qiangchuan Hou, Bilige Menhe and HepingZhang. High-throughput sequencing for the detection of the bacterial and fungaldiversity in Mongolian naturally fermented cow’s milk inRussia. BMC Microbiology , 2015,15:45(第一作者,SCI收录,IF=2.976)

6.Tong Dan,Wenjun Liu, Yuqin Song, HaiyanXu, Bilige Menghe, Heping Zhang and Zhihong Sun. The evolution and populationstructure ofLactobacillus fermentumfromdifferent naturally fermented products as determined by multilocus sequencetyping (MLST). BMC Microbiology (2015) 15:107 (共同第一作者) (共同第一作者,SCI收录,IF=2.976)

7.Tong Dan,Wenjun Liu, Zhihong Sun, QiangLv, Haiyan Xu, Yuqin Song and Heping Zhang A novel multi-locus sequence typing (MLST)protocol forLeuconostoc lactis isolates from traditional dairy products inChinaandMongolia. A novel multi-locussequence typing (MLST) protocol forLeuconostoc lactisisolates from traditionaldairy products inChinaandMongolia.BMC Microbiology, 2014,14:150. (共同第一作者SCI收录,IF=2.976)

8.Liu Wenjun, Xi Xiaoxia, Qi Muge Sudu,Guo Liru, Guo Zhang, Meng Hebilige, Sun Tiansong, Zhang Heping. High-throughputsequencing reveals microbial community diversity of Tibetan naturally fermentedyak milk.Annals of Microbiology, 2015 65(3):, 1741-175(第一作者,SCI收录,IF=0.99)

9.Liu Wen Jun, Y. F. Chen, L. Y. Kwok,M. H. Li, T. Sun, C. L. Sun, X. N. Wang, T. Dan, B. L. G. Menghe, H. P. Zhang,T. S. Sun.PreliminarySelection for Potential Probiotic Bifidobacterium Isolated from Subjects ofDifferent Chinese Ethnic Groups and Evaluation of Their Fermentation andStorage Characteristics in Bovine Milk. Journal of Dairy Science.J. Dairy Sci. 2013; 96 (11): 6807(第一作者,SCI收录,IF=2.573)

10.Haiyan Xu,Wenjun Liu, QimuGesudu, ZhihongSun, Jiachao Zhang, ZhuangGuo, Yi Zheng, QiangchuanHou, Jie Yu, Yanting Qing,Lai-Yu Kwok, BiligeMenhe and Heping Zhang. Assessment of the bacterial andfungal diversity in home-made yoghurts ofXinjiang,Chinabypyrosequencing. 2014, J Sci FoodAgric.2015,95(10): 2007–2015,(共同第一作者)(共同第一作者,SCI收录,IF=1.879)

11.Zhihong Sun,Wenjun Liu, Qiuhua Bao, JiachaoZhang, Qiangchuan Hou, Laiyu Kwok, Tiansong Sun, Heping Zhang. Investigation ofbacterial and fungal diversity in tarag using high-throughput sequencing. Journalof Dairy Science, 2014, 97(10): 6085–6096. (共同第一作者)(共同第一作者,SCI收录,IF=2.573)

12.W.J.Liu, Z.H. Sun, Y.B.Zhang, C.L. Zhang, M.H. Bilige, M. Yang, T.S. Sun, Q.H. Bao , W. Chen, H.P.Zhang. A survey of bacterial composition in kurut from Tibet of China using aculture-independent approach. Journal of Dairy Science. 2012, 95(3):1064-1072.(第一作者,SCI收录,IF=2.573)

13.WenjunLiu, Qiuhua Bao,Jirimutu, Manjun Qing, Siriguleng, Xia Chen, Ting Sun, Meihua Li, JiachaoZhang, Jie Yu, Menghe Bilige, Tiansong Sun, Heping Zhang.Isolationand Identification of Lactic Acid Bacteria from Tarag in Eastern Inner Mongoliaof China by 16S rRNA Sequences and DGGE analysis, Microbiological Research,2012, 167(2):110-115.(第一作者,SCI收录,IF=2.561)

14.LiuWen Jun, Li Hai yan,Chen Yong fu, Gao Wa, Liu Xiao ming, Zhang He ping. Analysis of chemicalcomposition, protein profile and microstructure of dairy fan in Yunnan ofChina,Milchwissenschaft, 2011, 66(1): 57-60(第一作者,SCI收录,IF=0.247)

15.WenjunLiu, Zhihong Sun, JiaochaoZhang, Wa Gao, Weihong Wang, Lan Wu, Tiansong Sun, Wei Chen, Xiaoming Liu,Heping Zhang. Analysis of microbial composition in acid whey for dairy fanmaking in Yunnan by conventional method and 16S rRNA sequencing, CurrentMicrobiology, 2009, 59: 199–205 .(第一作者,SCI收录,IF=1.429)

16.WenjunLiu, Jing Zeng, GonghaoJiang, Yuqing He. QTLs identification of crude fat content in brown rice andits genetic basis analysis using DH and two backcross populations. Euphytica,2009, 169: 197–205.(第一作者,SCI收录,IF=1.385)

17.Yongfu Chen,Wenjun Liu, Jiangang Xue, JieYang, Xia Chen, Yuyu Shao, Lai-yu Kwok, Menghe Bilige, Lai Mang, and HepingZhang Angiotensin-converting enzyme inhibitory activity of Lactobacillushelveticusstrains from traditional fermented dairy foods and antihypertensiveeffect of fermented milk of strain H9. J. Dairy Sci.2015, 97 :1–13

18.Jiachao Zhang,Wenjun Liu, Zhihong Sun, QiuhuaBao, Fang Wang, Jie Yu, Wei Chen*, and Heping Zhang*. Diversity of lactic acidbacteria and yeasts in traditional sourdoughs collected from western region inInner Mongolia of China. Food Control.2011, 22(5): 767-774. (SCI收录, IF: 2.812)

19.Wenyi Zhang,Wenjun Liu, Yuqing Song, HaiyanXu, Bilige Menghe, Heping Zhang, and Zhihong Sun. Multilocus sequence typing ofa dairy-associated Leuconostoc mesenteroides population reveals clonalstructure with intragenic homologous recombination. J. Dairy Sci.2015,TBC:1–10

20.Bilige, Menghe,Liu, Wenjun, Rina, Wu, Wang,Liping, Sun, Tiansong, Wang, Junguo, Li, Haiping, Zhang, Heping. Evaluation of potentialprobiotics properties of the screened Lactobacilli isolated from home-madekoumiss inMongolia.Annals of Microbiology, 59 (3), 493-498 (SCI)

21.Zhihong Sun,Wenjun Liu, Wa Gao, Mei Yang,Jiachao Zhang, Lan Wu, Junguo Wang, bilige Menghe, Tiansong Sun, and HepingZhang*,Identificationand characterization of the dominant lactic acid bacteria from kurut: thenaturally fermented yak milk in Qinghai, China,Journal of General andApplied Microbiology,2010,56:1-10

22.Zhihong Sun,Wenjun Liu, Jiachao Zhang JieYu, Cike Cai, Bilige Menghe, Tiansong Sun, and Heping Zhang* Identification andCharacterization of the dominant lactobacilli isolated from koumiss in ChinaJ. gen. Appl. Microbiol., 2010,56,257-265

23.Zhihong Sun,Wenjun Liu, Jiachao Zhang JieYu, Gao Wa, Mutu JiRi Bilige Menghe, Tiansong Sun, and Heping Zhang*Identification and characterization of the dominant lactic acid bacteria fromtraditional fermented milk in Mongalia.FoliaMicrobiol. 2010, 55 (3), 270-276

24.Jiachao Zhang,Wenjun Liu,Zhihong Sun, Qiuhua Bao, Fang Wang, Jie Yu, Wei Chen, and HepingZhang. Diversity of lactic acid bacteria and yeasts in traditional sourdoughscollected from western region in Inner Mongolia of China.Food Control. 2011, 22 (5): 767-774. (IF: 2.526)

25.Haiyan Xu, Zhihong Sun ,Wenjun Liu, Jie Yu, Yuqin Song,Qiang Lv, Jiachao Zhang, Yuyu Shao, Bilige menghe , and Heping Zhang. multilocussequence typing of Lactococcus lactisfrom naturally fermented milk foods inethnic minority areas of China. J. Dairy Sci. 2015, 97 :2633–2645

26.Jie Yu, Zhihong Sun,Wenjun Liu, Xiaoxia Xi, YuqinSong, Haiyan Xu, Qiang Lv, Qiuhua Bao, Bilige Menghe and Tiansong Sun.Multilocus sequence typing of Streptococcus thermophilus from naturallyfermented dairy foods in China and Mongolia

27.J. Yu, W. H. Wang, B. L. G.Menghe, M. T. Jiri, H. M. Wang,W. J.Liu, Q. H. Bao, Q. Lu, J. C. Zhang, F. Wang, H. Y. Xu, T. S. Sun, andH. P. Zhang. Diversity of lactic acid bacteria associated with traditionalfermented dairy products inMongolia.Journal of Dairy Science, 2011, 94(7): 3229-3241. (SCI收录, IF: 2.497)

28.Yang Mei, Chen Xia, Yu Jie,Liu Wenjun, Zhang Heping.Identification of Lactococci from Hogormag by Molecular Methods,农产食品科技, 2009, 3 (4): 46-50

29.Wang Junguo, Chen Xia,Liu Wenjun, Yang Mei, Airidengcaicike,Zhang Heping Identification of Lactobacillus from koumiss by conventional andmolecular methods.Eur Food Res Technol,2008, 227: 1555 – 1561 (SCI)

30.Jie Yu,Zhihong Sun,Wenjun Liu,Jiachao Zhang,Heping Zhang. Rapid identification of Lactic Acid Bacteria Isolatedfrom Home-made Fermented Milk in Tibet,2009,Journal of General and Applied Microbiology.55, 181-190. (SCI)

31.Jie Yu, Zhihong Sun,Wenjun Liu, Qiuhua Bao, JiachaoZhang, Heping Zhang. Phylogenetic study ofLactobacillus acidophilusgroup, L. caseigroup and L. plantarum group based on partial hsp60, pheS and tuf genesequences. Eur Food Res Technol. 2012, 234: 927–934.

32.Jie Yu, XiaoHua Du, WeiHongWang, Jiachao Zhang,Wenjun Liu,Zhihong Sun,Tiansong Sun, Heping Zhang,Phenotypic and genotypic characteristicsof Lactic acid bacteria isolated from Fermented Congee in Inner Mongolia ofChina, Journal of General and Applied Microbiology, 2011, 57: 197-206. (SCI收录,IF: 1.000)

33.Airidengcaicike, Xia Chen, XiaohuaDu, Weihong Wang, Jiachao Zhang, Zhihong Sun,WenjunLiu, LiLi,Tiansong Sun*, Heping Zhang, Isolation and Identification of CultivableLactic Acid Bacteria in Traditional Fermented Milk of Tibet in China,Int .J Dairy Techol. 2010,63 (3): 437-444

34.Yanyan Wang,Heping Zhang,Lei Zhang,Wenjun Liu,Yong Zhang,Xinchang ZhangTiansong Sun,In vitro assessment of probioticproperties of Bacillus isolated from naturally fermented congee from InnerMongolia of China,World Journal of Microbiology and Biotechnology, 2010, 26 (8): 1369-1377

35.Xia Chen,Mei Yang,Zhihong Sun,Wenjun Liu,Tiansong Sun, He Meng, Heping Zhang. Molecular Cloning andCharacterization of alpha subunit of H+-ATPase in Lactobacillus casei Zhang,Czech Journal of FoodSciences,2009, 27(1): 49-54. (SCI)

36.Zhihong Sun, Wenyi Zhang, ChenyiGuo, Xianwei Yang,Wenjun Liu,Yarong Wu, Yuqin Song, Lai Yu Kwok, Yujun Cui, Bilige Menghe, Ruifu Yang, LiangpingHu, Heping Zhang.Comparative GenomicAnalysis of 45 Type Strains of the Genus Bifidobacterium: A Snapshot of ItsGenetic Diversity and Evolution. PLOS ONE| DOI:10.1371/journal.pone.0117912

37.J. Yu, H. M. Wang, M. S.Zha, Y. T. Qing, N. Bai, Y. Ren, X. X. Xi,W.J. Liu, B. L. G. Menghe, and H. P. Zhang. Molecular identification andquantification of lactic acid bacteria in traditional fermented dairy foods ofRussia. J. Dairy Sci. 98:5143–5154.

38.L. Q. Wang,W. J. Liu,Y.Xu, Y. Q. He, L. J. Luo, Y. Z. Xing, C. G. Xu and Qifa Zhang. Gentic basis of17 traits and viscosity parameters characterizing the eating and cookingquality of rice grain.Theor Appl Genet.2007, 115: 463–476. (SCI)

39.刘文俊,乌日娜,张和平.益生菌L. caseiZhang的多项分类鉴定,中国乳品工业,2009,37(2):14-17

40.刘文俊,张和平.发酵乳中的主要风味物质及其代谢合成途径和关键功能基因.中国科技论文,2016,11(12):1391-1397

41.高娃,于洁,乌兰,孙志宏,刘文俊,孟和毕力格,孙天松,张和平*,蒙古国传统发酵乳中部分乳杆菌16SrRNA-RFLP分类研究,中国乳品工业,2010,38(1):4-7

42.刘文俊,王令强,何予卿.用两个重组自交系群体定位和比较控制水稻株高和抽穗期QTL.华中农业大学学报(自然科学版),2007,4(2):116-120

43.王炜宏,杜晓华,张家超,于洁,刘文俊;孙天松,张和平.内蒙古鄂尔多斯地区酸粥发酵液中乳酸菌的分离鉴定,食品与生物技术学报,2010, (2): 265-270

44.张春林,刘文俊,张彦斌,孙志宏,张和平,孙天松.西藏地区传统发酵乳宏基因组DNA的提取及微生物群落多样性分析,食品与发酵工业,2010,(4): 15-20

45.卿蔓君,白梅,张勇,刘文俊,孙志宏,张和平,孙天松.西藏曲拉和云南乳饼中酵母菌的鉴定及其生物多样性.微生物学报, 2010, 9: 1141-1146

46.王炜宏,杜晓华,张家超,于洁,刘文俊,孙天松,张和平,内蒙古鄂尔多斯地区酸粥发酵液中乳酸菌的分离鉴定.食品与生物技术学报,2010,29(2):265-270

47.高娃,于洁,乌兰,孙志宏,刘文俊,孟和毕力格,孙天松,张和平*.蒙古国传统发酵乳中部分乳杆菌16SrRNA-RFLP分类研究,中国乳品工业,2010,38(1):4-7

48.张家超,王芳,徐海燕,于洁,刘文俊,包秋华,孙志宏,张和平. 6种区分乳酸乳球菌亚种和乳酸乳球菌乳脂亚种的分子生物学方法比较,微生物学报,2010,50(12):1670-1676

49.张家超,孙志宏,刘文俊,张和平,适用于乳酸菌分类鉴定的分子生物学技术,乳业科学与技术,2009,2:89-93.

50.赵文静,刘文俊,李妍,孙志宏,张和平.乳酸菌定量分析方法研究进展,食品与发酵工业,2009,35,121-125.

51.张家超,孙志宏,刘文俊,张和平.适用于乳酸菌分类鉴定的分子生物学技术,乳业科学与技术, 2009, 2: 89-93.

52.剧柠,张家超,孙志宏,刘文俊,靳烨,张和平,孙天松.藏北地区传统发酵乳中乳杆菌的多样性分析.微生物学报, 2009, 49 (11): 1540-1547

53.陈永福,刘文俊,包秋华,张和平,高效液相色谱法测定传统发酵乳维生素C含量,乳业科学与技术,2008(1):9-11

出版专著

1.张和平主编,刘文俊,孙志宏,张文羿,陈永福,包秋华参编.自然发酵乳制品中乳酸菌生物多样性(华夏英才基金学术文库),2012年1月,科学出版社. P: 34-99, 110-200.

2.Liu W.J. Pang H. L., Zhang H.P., CaiY.M. 2014, In: Hepng Zhang Yimin Cai (ed.), Lactic Acid Lactic Acid BacteriaFundamentals and Practice, Chapter 2, p: 85-110, Springer. ISBN978-94-017-8840-3

出版教材

1.1).曾凡坤、张玉(主编),刘文俊等(参编).农产品加工贮运学实验(“十三五农林规划教材”).中国农业出版社,2016年4月。

授权专利

1.张和平,包秋华,刘文俊.益生菌乳制品中鼠李糖乳杆菌的快速定性、定量检测方法;专利号:ZL2009101779110;授权日:2012年01月11日.

2.张和平,刘文俊,张家超.益生菌乳制品中植物乳杆菌的快速定性、定量检测方法,专利号:ZL 2009101779089;授权日:2012年01月25日.

3.张和平,孙志宏,张家超,刘文俊,包秋华.一种适合乳酸菌16SrRNA序列测定的引物设计:专利号:ZL200910250674;授权日:2012年4月18日.

4.张和平,张家超,刘文俊,包秋华,孙志宏.发明名称:一种发酵乳制品中乳酸菌种类快速检测的方法;专利:ZL 2009 1 0250669.5;授权日:2012年05月30日;

获得奖励

1.张和平,张文羿,孙志宏,王记成,刘文俊,陈永福,包秋华.“益生菌Lactobacillus caseiZhang—从基础研究到产业化开发”,2011年度教育部高等学校科技进步二等奖,2011年11月。

鉴定科研成果

1.张和平,刘文俊,孟和毕力格,孙天松,吉日木图,包秋华,于洁,张家超,孙志宏,张文羿,陈霞,乌日娜. 2010年.自然发酵乳制品(及其它发酵食品)中乳酸菌生物多样研究及乳酸菌菌种资源库建设.内蒙古自治区科技成果鉴定(内科鉴字2010第62号)。

2.孙天松,刘文俊,张文羿,乌日娜,王记成,卿曼君,吴斯日古冷. 2012年,自然发酵乳制品中乳杆菌耐酸、耐胆盐关键候选基因表达。内蒙古自治区科技成果鉴定(证书编号:NK-20120374

3.张和平,孟和,陈卫,张文羿,乌日娜,孙志宏,王俊国,孟和毕力格,包秋华,刘文俊,陈永福.2008年.益生菌L. casei Zhang基因组学和蛋白质组学研究.年内蒙古自治区科技成果鉴定(内科鉴字2008第50号)。

4.张和平,孟和毕力格,孙天松,王俊国,孙志宏,刘文俊,包秋华,陈永福,钟智,张文羿,赵文静. 2011年.乳酸菌与发酵乳制品开发基础研究.内蒙古自治区科技成果鉴定(成果登记号:NK-20110256)。

5.张和平,张文羿,孙志宏,王记成,刘文俊,陈永福,包秋华. 2011年.益生菌Lactobacillus caseiZhang—从基础研究到产业化开发.教育部科技成果鉴定(成果登记号:360-11-27150813-05)。

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